abstract：:The extent of lipoprotein glycation was assessed in 82 patients diagnosed as diabetes mellitus type II. Glycated lipoprotein levels in serum were determined by agarose gel film electrophoresis in 48 non-diabetic control subjects, 56 diabetics with complications and 26 diabetics with no complications. All glycated lipo...

journal_title：Annals of clinical biochemistry

authors： Yeğin A,Ozben T

更新日期：1995-09-01 00:00:00

abstract：:In monocytes at the secretory (oestrogen-progesterone dominant) phase of the menstrual cycle, expression of c-fms and macrophage colony-stimulating factor (M-CSF) receptor and activity of tyrosine kinase (TK) were increased by oestradiol with or without progesterone. In vivo, oestrogen may induce expression of c-fms a...

journal_title：Annals of clinical biochemistry

authors： Fujimoto J,Hori M,Ichigo S,Itoh T,Saio M,Takami T,Tamaya T

更新日期：1995-07-01 00:00:00

abstract：:591 patients with a history of coronary heart disease had one or more biochemical markers of tobacco smoking measured. 26% were self reported smokers and a further 4% were apparent 'smoking deceivers'. The urinary nicotine metabolite concentration is an excellent marker for tobacco smoking; breath CO would be a suitab...

journal_title：Annals of clinical biochemistry

authors： Archbold GP,Cupples ME,McKnight A,Linton T

更新日期：1995-03-01 00:00:00

abstract：:A comparison of the Roche Cobas Core and Hybritech TANDEM-E PSA (prostate specific antigen) assays revealed a large difference in the reactivity of each assay to the separated free and complexed forms of serum PSA in patients with prostatic carcinoma. The Roche assay was relatively much more responsive to the free for...

journal_title：Annals of clinical biochemistry

authors： Tillyer CR,Konings M,Gobin PT,Iqbal J

更新日期：1994-09-01 00:00:00

abstract：:Measurement of urine-free (unconjugated) cortisol (UFC) excretion is widely used in the investigation of hypercortisolaemia. We have measured 24 h UFC excretion in normal healthy individuals using a radioimmunoassay (RIA) method claimed to be suitable for unextracted urine. Significantly higher rates of excretion were...

journal_title：Annals of clinical biochemistry

authors： Lamb EJ,Noonan KA,Burrin JM

更新日期：1994-09-01 00:00:00

abstract：:Total serum cholesterol was measured in 1320 normal Saudi children aged 0-14 years. The result was 3.88 (0.83) mmol/L [mean (SD)] and there was no statistical difference between girls and boys. Results were lowest in the 0-4 year age group and highest in the 5-9 year age group. Percentile values were established for t...

journal_title：Annals of clinical biochemistry

authors： Rafii ZS,al Nasser AA,Budair A,Tufail M

更新日期：1994-07-01 00:00:00

abstract：:Salivary theophylline concentrations in neonates were measured by a homogeneous enzyme immunoassay and compared with serum concentrations. Free serum theophylline concentrations were also compared with salivary concentrations and the values obtained were almost identical. Results using the immunoassay agreed well with...

journal_title：Annals of clinical biochemistry

authors： Thompson SJ

更新日期：1994-05-01 00:00:00

abstract：:The effects of inhibition of inosine monophosphate dehydrogenase (IMPDH), the rate-limiting enzyme in guanine nucleotide de novo synthesis, on cell growth, cell viability, endogenous nucleotide concentrations and concentrations of extracellular nucleosides and bases were studied in Molt F4 human malignant lymphoblasts...

journal_title：Annals of clinical biochemistry

authors： Stet EH,De Abreu RA,Bökkerink JP,Lambooy LH,Vogels-Mentink TM,Keizer-Garritsen JJ,Trijbels FJ

更新日期：1994-03-01 00:00:00

abstract：:The number of known inherited metabolic disorders resulting in an organic aciduria has increased steadily over the past two decades. Prompt and reliable detection is both clinically and technically demanding but is essential if appropriate treatment is to be undertaken. This is the first study of laboratory performanc...

journal_title：Annals of clinical biochemistry

authors： Bonham JR,Downing M,Pollitt RJ,Manning NJ,Carpenter KH,Olpin SE,Allen JC,Worthy E

更新日期：1994-03-01 00:00:00

abstract：:The seasonal variation of serum 25-hydroxy vitamin D3 and 1,25-dihydroxy-vitamin D has been investigated. Blood was taken from 27 healthy volunteers, aged 21-44 years old at 3 monthly intervals over a period of 1 year. A scrolling monthly programme with 12 quarterly (3 month) time periods was developed. A summer assoc...

journal_title：Annals of clinical biochemistry

authors： Hine TJ,Roberts NB

更新日期：1994-01-01 00:00:00

abstract：:A study of conditions for the elution of apo A1 and B lipoproteins from dried blood spots has led to the development of an apo B/A1 ratio assay with results for dried blood spots which are comparable with serum assays. This assay has been developed to be suitable for large scale population screening. The concept of me...

journal_title：Annals of clinical biochemistry

authors： Hirst AD,Beswick K

更新日期：1993-09-01 00:00:00

abstract：:Serum total sialic acid (TSA) has gained medical interest, particularly as a cardiovascular risk factor and it has been hypothesized that serum levels relate to serum acute phase proteins, some of which are sialylated. We assayed serum TSA and also lipid associated sialic acid (LASA) in 50 normal individuals (24 male)...

journal_title：Annals of clinical biochemistry

authors： Haq M,Haq S,Tutt P,Crook M

更新日期：1993-07-01 00:00:00

abstract：:We have evaluated the clinical relevance of monitoring acute phase proteins in severe urinary tract infection. Body temperature, white blood cell count, erythrocyte sedimentation rate, serum amyloid A protein (SAA), C-reactive protein (CRP), alpha-1-antichymotrypsin (ACT) and alpha-1-acid glycoprotein (AGP) were deter...

journal_title：Annals of clinical biochemistry

authors： Casl MT,Sabljar-Matovinović M,Kovacević S,Pocanić D,Preden-Kereković V,Jagarinec N

更新日期：1993-05-01 00:00:00

abstract：:A sensitive immunochemiluminometric assay with a detection limit of 1.1 microU/L was developed for the measurement of urinary growth hormone (UGH). The assay was shown to be specific and precise. There was a good correlation between serum growth hormone (GH) and UGH concentrations in 20 patients with acromegaly and si...

journal_title：Annals of clinical biochemistry

authors： Turner G,Brown RC,Weeks I,Butler GE,Creagh FN,Woodhead JS

更新日期：1993-03-01 00:00:00

abstract：:The diagnostic performance of a new enzyme linked immunosorbent assay for the cardiac structural protein troponin T in the differential diagnosis of ischaemic cardiac damage was assessed. A well documented set of patients admitted to the coronary care unit of a district general hospital were studied. At a cutoff value...

journal_title：Annals of clinical biochemistry

authors： Collinson PO,Moseley D,Stubbs PJ,Carter GD

更新日期：1993-01-01 00:00:00

abstract：:Serum amyloid A protein (SAA) is a sensitive acute phase reactant. We developed a method for the rapid measurement of human SAA in serum by kinetic nephelometry of anti-SAA antibody-coated latex agglutination. Measurement takes less than 6 min using an automated analyser. Standardization of the assay employs SAA-enric...

journal_title：Annals of clinical biochemistry

authors： Yamada T,Nomata Y,Sugita O,Okada M

更新日期：1993-01-01 00:00:00

abstract：:The concentrations of serum calcium, parathyroid hormone (PTH), 25 Hydroxyvitamin D (25OHD), and 1,25 Dihydroxyvitamin D (1,25(OH)2D) were determined in 99 Saudi patients with sickle cell disease and in 104 matching healthy controls. Serum calcium and 25OHD were significantly lower in the patients, with 14% and 12% of...

journal_title：Annals of clinical biochemistry

authors： Mohammed S,Addae S,Suleiman S,Adzaku F,Annobil S,Kaddoumi O,Richards J

更新日期：1993-01-01 00:00:00

abstract：:Quadriceps muscle specimens from autopsy of 28 neonates (gestational age 25-42 weeks) were investigated to determine pyruvate and malate oxidation rates and several enzymes of the mitochondrial oxidative process. In general, the levels of all mitochondrial parameters measured, including carnitine levels, were lower in...

journal_title：Annals of clinical biochemistry

authors： Sperl W,Sengers RC,Trijbels JM,Ruitenbeek W,Doesburg WH,Smeitink JA,Kollée LA,Boon JM

更新日期：1992-11-01 00:00:00

abstract：:A rapid vacuum-driven procedure, using pre-treated Sep-Pak C18 cartridges, has been developed for the simultaneous extraction of arginine vasopressin (AVP) and atrial natriuretic peptide (ANP) from plasma. Non-specific interference was removed by fractional elution with an aqueous methanol/trifluoroacetic acid (TFA) m...

journal_title：Annals of clinical biochemistry

authors： Penney MD,Hampton D,Oleesky DA,Livingstone C,Mulkerrin E

更新日期：1992-11-01 00:00:00

abstract：:The measurement of glycated haemoglobin (glycated Hb) by automated high pressure liquid chromatography enabled the prevalence of haemoglobin variants (Hb variant) to be determined in a large diabetic population within Leeds, UK. There were 16 patients showing a Hb variant amongst a total of 5300 diabetics, the majorit...

journal_title：Annals of clinical biochemistry

authors： Allen KR,Hamilton AD,Bodansky HJ,Poon P

更新日期：1992-07-01 00:00:00

abstract：:A microtiter plate based Dual Analyte enzyme-immunoassay method for the simultaneous measurement of alpha-fetoprotein (AFP) and Free-beta human chorionic gonadotrophin (hCG) was evaluated. This rapid assay, which has application in both Neural Tube Defect screening and Down's screening, shows good precision with betwe...

journal_title：Annals of clinical biochemistry

authors： Macri JN,Spencer K,Anderson R

更新日期：1992-07-01 00:00:00

abstract：:The hypoglycaemia caused by non-islet cell tumours is often associated with an increase in plasma insulin-like activity. In many cases there is a relative if not always absolute increase in plasma insulin-like growth factor II (IGF-II). Growth hormone (GH) secretion is almost invariably depressed as is the plasma insu...

journal_title：Annals of clinical biochemistry

authors： Teale JD,Blum WF,Marks V

更新日期：1992-05-01 00:00:00

abstract：:A binding assay for sex hormone binding globulin (SHBG) has been developed in which SHBG is saturated with tritiated dihydrotestosterone (DHT). Separation of bound and free DHT is achieved by using Concanavalin A-Sepharose as a solid phase matrix. The method is described and its performance, including linearity, impre...

journal_title：Annals of clinical biochemistry

authors： Whittaker JA,Cawood ML,Oakey RE

更新日期：1992-03-01 00:00:00

abstract：:The present study was designed to re-evaluate the radioimmunoassay for desmosine in urine, which is currently used as a measure of elastin metabolism. Using ion exchange chromatography, gel filtration and affinity chromatography it was shown that at least five other compounds in hydrolysates of human urine competed fo...

journal_title：Annals of clinical biochemistry

authors： Starcher B,Scott M

更新日期：1992-01-01 00:00:00

abstract：:Plasma C1 inhibitor (C1-INH) was determined in 64 patients with different malignant diseases and in 58 healthy persons. C1-INH antigen concentration was measured by radial immunodiffusion (RID), whereas its functional activity was assayed with chromogenic substrate. Within-run and day-to-day precision of both methods ...

journal_title：Annals of clinical biochemistry

authors： Starcević D,Jelić-Ivanović Z,Kalimanovska V

更新日期：1991-11-01 00:00:00

abstract：:A recently introduced turbidimetric immunoassay using shell/core particles for determination of myoglobin (Turbiquant Myoglobin, Behringwerke, Marburg, Germany) was evaluated on the Behring Turbitimer analyzer. Intra-assay CV varied between 1.0% and 3.2%; interassay CV was between 2.0% and 3.6%. Linear results were ob...

journal_title：Annals of clinical biochemistry

authors： Delanghe J,Chapelle JP,el Allaf M,De Buyzere M

更新日期：1991-09-01 00:00:00

abstract：:The Bhattacharya method and the 'average of normals' method for internal quality control were compared. Both are based on unselected patient test results. The Bhattacharya method is mostly used in clinical chemistry for calculation of reference intervals but could be modified for quality control procedures. Using the ...

journal_title：Annals of clinical biochemistry

authors： Oosterhuis WP,Modderman TA,Dinkelaar RB,Zwinderman AH,van der Helm HJ

更新日期：1991-07-01 00:00:00

abstract：:A previously described method for mannitol in urine has been modified and improved. End product inhibition by fructose in the mannitol dehydrogenase method for mannitol has been minimized; the assay is linear over a sample mannitol concentration range of 0-12 mmol/L; no significant interference from other sugars or su...

journal_title：Annals of clinical biochemistry

authors： Blood J,Ingle AR,Allison N,Davies GR,Hill PG

更新日期：1991-07-01 00:00:00

abstract：:Rabbit uterus contains type I and II oestrogen binding sites in the cytosol, and nuclear fractions. Oestrogen-stimulated increase in uterine weight was inhibited by concurrent treatment with progesterone or danazol. Oestrogen-induced type I binding sites were decreased by progesterone and to a lesser extent by danazol...

journal_title：Annals of clinical biochemistry

authors： Tamaya T,Wada K,Mori H,Imai A

更新日期：1991-05-01 00:00:00

abstract：:The most widely used methods for the assay of total bilirubin in serum are based on a coupling reaction with various diazo dyes in the presence of an accelerating agent. The 'direct' reaction, without accelerator, gives a good estimate of the conjugated and protein bound (delta) species of bilirubin only if carefully ...

journal_title：Annals of clinical biochemistry

authors： Westwood A

更新日期：1991-03-01 00:00:00

abstract：:Serum osteocalcin did not show any response to the onset of osteosarcoma in Paget's disease of bone whereas serum alkaline phosphatase increased rapidly. This suggests that osteocalcin is not useful in the diagnosis and management of Paget's osteosarcoma and does not reflect the same osteoblastic processes in bone as ...

journal_title：Annals of clinical biochemistry

authors： Davie MW,Worsfold M,Sharp CA

更新日期：1991-03-01 00:00:00

abstract：:In a 1 year prospective study we evaluated the effect of feedback of laboratory data on the requesting behaviour of physicians in general medicine. Data on within-hours and out-of-hours clinical chemistry laboratory usage and revenue expenditure for inpatients and outpatients, expressed in terms of clinical workload, ...

journal_title：Annals of clinical biochemistry

authors： Gama R,Nightingale PG,Broughton PM,Peters M,Bradby GV,Berg J,Ratcliffe JG

更新日期：1991-03-01 00:00:00

abstract：:This paper reviews the physiology and pathology of oxygen transport from the atmosphere to the cells in critically ill patients. A thorough understanding of this allows a rational approach to monitoring and managing an hypoxic patient in the intensive care setting. ...

journal_title：Annals of clinical biochemistry

authors： Clapham MC

更新日期：1991-01-01 00:00:00

abstract：:We compared the diagnostic utility of recently proposed slope assays for serum creatine kinase and creatine kinase-2 with the optimized decision threshold assays. The former approach has been claimed to be superior to any other single diagnostic technique. We show, by ROC curve and likelihood ratio analyses, that the ...

journal_title：Annals of clinical biochemistry

authors： Leung FY,Griffith AP,Jablonsky G,Henderson AR

更新日期：1991-01-01 00:00:00

abstract：:The mechanisms of hypercalcaemia were assessed in 20 hypercalcaemic patients with breast cancer. Abnormalities suggestive of a PTH-related peptide (PTHrP) mechanism were observed in up to 60% of cases; urinary cyclic adenosine monophosphate (UcAMP) was elevated in nine patients (45%), renal tubular reabsorption of cal...

journal_title：Annals of clinical biochemistry

authors： Gallacher SJ,Fraser WD,Patel U,Logue FC,Soukop M,Boyle IT,Ralston SH

更新日期：1990-11-01 00:00:00

abstract：:The effect on the concentrations of specific serum proteins and tumour markers, of heat-treating samples at 56 degrees C for 30 min to inactivate HIV, was investigated. Statistically significant decreases, that may affect clinical decisions, were observed for alpha-1-antitrypsin, beta-2-microglobulin, IgE, fibrinogen,...

journal_title：Annals of clinical biochemistry

authors： Gleeson M,Herd L,Burns C

更新日期：1990-11-01 00:00:00

abstract：:We investigated the effect of a fatty meal on plasma concentrations of lipids, apolipoproteins, and the cholesterol component of lipoproteins. Sixteen nonobese, healthy, asymptomatic males, 22-34 years of age, served as subjects for this study. None smoked, consumed more than two alcoholic drinks per day, or took any ...

journal_title：Annals of clinical biochemistry

authors： Rifai N,Merrill JR,Holly RG

更新日期：1990-09-01 00:00:00

abstract：:The Poser criteria for diagnosing multiple sclerosis (MS) includes clinical, paraclinical and laboratory information. We studied the influence of cerebrospinal fluid (CSF) biochemistry results on the categorisation of patients with suspected MS. A retrospective study was made of 138 patients who had CSF samples sent o...

journal_title：Annals of clinical biochemistry

authors： Ratnaike S,Kilpatrick T,Tress B,Davis S,Kilpatrick C,Byron K,Deam D

更新日期：1990-05-01 00:00:00

abstract：:A series of experiments are described which show that second derivative spectroscopy can be used to quantify conjugated lipid dienes as markers of lipid peroxidation in heptane extracts of plasma from patients with rheumatoid arthritis, osteoarthritis, and healthy controls. Results obtained by this method gave reasona...

journal_title：Annals of clinical biochemistry

authors： Situnayake RD,Crump BJ,Zezulka AV,Davis M,McConkey B,Thurnham DI

更新日期：1990-05-01 00:00:00

abstract：:The spectral interference caused by haemoglobin in the calcium-cresolphthalein complexone reaction was investigated. The results show that the nature of the haemoglobin derivative in the reaction mixture influences the degree of interference; in turn, the derivative formed depends on the order in which the sample and ...

journal_title：Annals of clinical biochemistry

authors： Corns CM

更新日期：1990-03-01 00:00:00